The DMPO Antibody: HRP from MyBioSource.com is a Mouse Monoclonal antibody. This antibody recognizes Human, Mouse, Rat, Amoeba/Protozoa, Bacteria/Archaea, Bovine, C. elegans/Worm, Canine, Chicken/Bird, Donkey, Drosophila/Arthropod, Feline, Goat, Guinea Pig, Hamster, Horse, Mollusc, Non-Human Primate, Plant, Porcine, Reptile, Rabbit, Sheep, Virus, Xenopus/Amphibian, Yeast/Fungi, Zebrafish/Fish, Other Invertebrate, and Other Mammalian antigen. The DMPO Antibody: HRP has been validated for the following applications: ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot, and Other.
Description
Background Info: Detects ~90kDa. Recognzies DMPO, DMPO-octanoic acid, DMPO-protein adducts and DMPO-DNA adducts. Does not cross react with non-adducted proteins or DNA.
Scientific Background: The formation of free radicals and other highly reactive oxygen species has been implicated in the pathogenesis of many disease states (1). The ability to identify these species is crucial, and spin trapping has accomplished this goal. DMPO (5,5-dimethyl-1-pyrroline N-oxide) is one of the least toxic to cells and animals, and possesses convenient pharmacokinetics (uptake, distribution, metabolism and excretion) in biological systems (2-6). Recent studies have determined that nitric oxide may substantially affect the quantitative determination of DMPO adducts, and therefore extra caution is required when studying generation of these species in the presence of nitric oxide or its radicals (1). DMPO adducts can be generated with protein and DNA radicals (7)